Researchers in the Blavatnik Institute at Harvard Medical School (HMS) and the Biological Research Center in Szeged, Hungary, say they have developed a new genetic engineering technique that promises to transform recombineering methodology.
Their report “Improved bacterial recombineering by parallelized protein discovery” is published in PNAS.
The team created a high-throughput screening technology to look for the most efficient proteins that serve as the engines of recombineering. Such single-stranded DNA-annealing proteins (SSAPs) reside within phages.
