Spatial biology’s technological advances over the past few years have deepened researchers’ ability to ask biological questions. However, limitations remain. For example, methods are lacking for the molecular analysis of large biological specimens imaged in 3D.
Now, a novel spatial technique, DISCO-MS, combines whole-organ/whole-organism clearing and imaging, deep-learning-based image analysis, robotic tissue extraction, and ultra-high-sensitivity mass spectrometry to enable unbiased proteome analysis of preclinical and clinical tissues after unbiased imaging of entire specimens in 3D.
